Sepax Technologies, Inc.

Sepax Polar-Pyridine Columns

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Application

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Utilizing highest purity and enhanced mechanical stability silica and pure bonding reagents, Polar-Pyridine phase is bonded with ethyl pyridine functional groups. With a polar and N-containing ring structure, Polar-Pyridine phase is slightly basic and adequate for many applications, such as pharmaceuticals, peptides, sugars, and nucleotides.

Silica:	Spherical, high purity (<10 ppm metals)
Pore size:	120 Å
Particle size:	1.8, 2.2, 3, 5 and 10 µm
Pore volume:	1.0 ml/g
Surface area:	300 m²/g
Phase structure:	Polymeric and fully endcapped
% Carbon:	8.0%
Coverage:	~ 3.3 µmol/m²

Well controlled chemistry of polymeric monolayer
High column-to-column reproducibility
Analytical, semi-preparative, and preparative separation
HILIC phase for separations of acidic, neutral and basic organic compounds, as well as pharmaceuticals

Polar-Pyridine

1.8 µm

2.2 µm

3 µm

5 µm

10 µm

Sepax Polar-Pyridine

Standard Dimension:

Part Number	Particle Size	Pore Size	ID×Length

134251-4605	1.8 µm	120 Å	4.6×50mm
134251-4610	1.8 µm	120 Å	4.6×100mm
134251-4615	1.8 µm	120 Å	4.6×150mm

Custom Dimension:

Part Number	Particle Size	Pore Size	ID×Length

134251-2103	1.8 µm	120 Å	2.1×30mm
134251-2105	1.8 µm	120 Å	2.1×50mm
134251-2110	1.8 µm	120 Å	2.1×100mm
134251-2115	1.8 µm	120 Å	2.1×150mm

Additional dimension upon request.

Coupler
P/N: 102002-COUPLER
SS with PEEK ferrule inserts, recommended to connect columns in series.
price: $75/ea

Sepax Polar-Pyridine

Standard Dimension:

Part Number	Particle Size	Pore Size	ID×Length

134252-4605	2.2 µm	120 Å	4.6×50mm
134252-4610	2.2 µm	120 Å	4.6×100mm
134252-4615	2.2 µm	120 Å	4.6×150mm

Custom Dimension:

Part Number	Particle Size	Pore Size	ID×Length

134252-2103	2.2 µm	120 Å	2.1×30mm
134252-2105	2.2 µm	120 Å	2.1×50mm
134252-2110	2.2 µm	120 Å	2.1×100mm
134252-2115	2.2 µm	120 Å	2.1×150mm

Additional dimension upon request.

Coupler
P/N: 102002-COUPLER
SS with PEEK ferrule inserts, recommended to connect columns in series.
price: $75/ea

Sepax Polar-Pyridine

Standard Dimension:

Part Number	Particle Size	Pore Size	ID×Length

134253-4605	3 µm	120 Å	4.6×50mm
134253-4615	3 µm	120 Å	4.6×150mm
134253-4625	3 µm	120 Å	4.6×250mm

Custom Dimension:

Part Number	Particle Size	Pore Size	ID×Length

134253-2001 ^[1]	3 µm	120 Å	2.0×10mm
134253-2001C ^[2]	3 µm	120 Å	2.0×10mm
134253-2103	3 µm	120 Å	2.1×30mm
134253-2105	3 µm	120 Å	2.1×50mm
134253-2115	3 µm	120 Å	2.1×150mm
134253-2125	3 µm	120 Å	2.1×250mm
134253-4001 ^[1]	3 µm	120 Å	4.0×10mm
134253-4001C ^[2]	3 µm	120 Å	4.0×10mm

Additional dimension upon request.

[1]: Guard Cartridge only.
[2]: Guard Cartridge with holder (Part number ended with "C").

Precolumn Filters (Alternative choice for Guard Cartridge)
P/N#: 102000-P355 (2 µm PEEK frit), $65.00/ea
- for column with particle size 5 µm and 10 µm.
P/N#: 102000-P356 (0.5 µm PEEK frit), $65.00/ea
- for column with particle size 3 µm, or less than 3 µm.

PEEK Refill Frits (For Precolumn Filter)
P/N#: 102001-P355, 2 µm, 5 units/pk, $100/pk
P/N#: 102001-P356, 0.5 µm, 5 units/pk, $100/pk

Coupler
P/N: 102002-COUPLER
SS with PEEK ferrule inserts, recommended to connect columns in series.
price: $75/ea

Sepax Polar-Pyridine

Standard Dimension:

Part Number	Particle Size	Pore Size	ID×Length

134255-4605	5 µm	120 Å	4.6×50mm
134255-4615	5 µm	120 Å	4.6×150mm
134255-4625	5 µm	120 Å	4.6×250mm
134255-10015	5 µm	120 Å	10.0×150mm
134255-10025	5 µm	120 Å	10.0×250mm
134255-21201 ^[1]	5 µm	120 Å	21.2×10mm
134255-21205	5 µm	120 Å	21.2×50mm
134255-21215	5 µm	120 Å	21.2×150mm
134255-21225	5 µm	120 Å	21.2×250mm
134255-30005	5 µm	120 Å	30.0×50mm
134255-30015	5 µm	120 Å	30.0×150mm
134255-30025	5 µm	120 Å	30.0×250mm

Custom Dimension:

Part Number	Particle Size	Pore Size	ID×Length

134255-2001 ^[1]	5 µm	120 Å	2.0×10mm
134255-2001C ^[2]	5 µm	120 Å	2.0×10mm
134255-2103	5 µm	120 Å	2.1×30mm
134255-2105	5 µm	120 Å	2.1×50mm
134255-2115	5 µm	120 Å	2.1×150mm
134255-2125	5 µm	120 Å	2.1×250mm
134255-4001 ^[1]	5 µm	120 Å	4.0×10mm
134255-4001C ^[2]	5 µm	120 Å	4.0×10mm

Additional dimension upon request.

[1]: Guard Cartridge only.
[2]: Guard Cartridge with holder (Part number ended with "C").

PEEK Refill Frits (For Precolumn Filter)
P/N#: 102001-P355, 2 µm, 5 units/pk, $100/pk
P/N#: 102001-P356, 0.5 µm, 5 units/pk, $100/pk

Coupler
P/N: 102002-COUPLER
SS with PEEK ferrule inserts, recommended to connect columns in series.
price: $75/ea

Sepax Polar-Pyridine

Standard Dimension:

Part Number	Particle Size	Pore Size	ID×Length

134259-10015	10 µm	120 Å	10.0×150mm
134259-10025	10 µm	120 Å	10.0×250mm
134259-21201 ^[1]	10 µm	120 Å	21.2×10mm
134259-21205	10 µm	120 Å	21.2×50mm
134259-21215	10 µm	120 Å	21.2×150mm
134259-21225	10 µm	120 Å	21.2×250mm
134259-30005	10 µm	120 Å	30.0×50mm
134259-30015	10 µm	120 Å	30.0×150mm
134259-30025	10 µm	120 Å	30.0×250mm

Custom Dimension:

Part Number	Particle Size	Pore Size	ID×Length

134259-4001 ^[1]	10 µm	120 Å	4.0×10mm
134259-4001C ^[2]	10 µm	120 Å	4.0×10mm

Additional dimension upon request.

[1]: Guard Cartridge only.
[2]: Guard Cartridge with holder (Part number ended with "C").

PEEK Refill Frits (For Precolumn Filter)
P/N#: 102001-P355, 2 µm, 5 units/pk, $100/pk
P/N#: 102001-P356, 0.5 µm, 5 units/pk, $100/pk

Coupler
P/N: 102002-COUPLER
SS with PEEK ferrule inserts, recommended to connect columns in series.
price: $75/ea

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