Ion-exchange Resins

Using porous polyacrylate particle as the support, Generik MC-SCX, WCX, SAX and WAX ion-exchange media are synthesized. Generik MC-SCX is a strong cation exchanger with sulfonate functional group. Generik MC-WCX is a weak cation exchanger with carboxylate functional group. Generik MC-SAX is a strong anion exchanger with quaternary ammonium functional group. Generik MC-WAX is a weak anion exchanger with tertiary amine functional group. The controlled manufacturing process guarantees high batch-to-batch reproducibility. Their high capacity and high mechanical strength enable those media ideal for preparative separation and purification of proteins, peptides, nucleic acids, antibodies, and other biopharmaceuticals.

Typical Properties

Products		Generik MC-SAX	Generik MC-SCX	Generik MC-WAX	Generik MC-WCX
Ion-exchanger type		Strong anion	Strong cation	Weak anion	Weak cation
Functional group		-N+(CH3)3	-SO3-	-HN+ (C2H5)2	-CH2COO-
Ionic capacity (meq/mL)		0.32-0.37	0.18-0.22	0.15-0.21	0.14-0.19
Average particle size (µm)		30, 60	30, 60	30, 60	30, 60
Average pore size (Å)		800	800	800	800
Storage solvent		20% ethanol	20% ethanol	20% ethanol	20% ethanol
DBC*		45 mg/mL	42 mg/mL	32 mg/mL	33 mg/mL
Chemical stability		Commonly used aqueous buffers, such as phosphate, Tris, acetate.
Cleaning solutions		0.5 M HCl, 0.5 M NaOH
pH stability		2~13	2~13	2~13	2~13
Volume change	pH 2-13	<1%	<1%	<1%	<1%
	0.01-1.0M NaCl	<1%	<1%	<1%	<1%
Regeneration salt		1-2 M NaCl	1-2 M NaCl	1-2 M NaCl	1-2 M NaCl

*DBC (Dynamic Binding Capacity) measurement method: for Generik MC-SAX and Generik MC-WAX, 10.0 mg/mL of BSA in 50 mM Tris buffer, pH 8.7, column size 10×150 mm, linear flow rate 212 cm/h, 10% breakthrough. For Generik MC-SCX and Generik MC-WCX, 10.0 mg/mL of lysozyme in 50 mM phosphate buffer, pH 6.0, column size 10×150 mm, linear flow rate 230 cm/h, 10% breakthrough.

• High chemical stability for low leaching
• Spherical particles with narrow particle size distribution
• High accessible surface area and high loading capacity
• High retentativity and selectivity
• Wide pH applications (pH=2-13)
• High lot to lot consistency
• Available from grams to multi-Kilogram

• Purification of proteins, peptides, proteins, nucleic acids, oligonucleotides and antibodies
• Suitable for heparin purification
• Solid phase extraction
• Flash chromatography
• Process purification