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MonomixTM IEX

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Ion exchange (IEX) chromatography enables the separation of native biological samples without disruption of high order structures. It has been widely used in the analysis and separation of ionizable pharmaceutical molecules. The method can be applied to all stages of the biological sample purification process. Currently commercially available IEX media are mostly based soft agarose and polymethacrylate, which only accommodates low pressure operation. Sepax has developed alternative polymethacrylate-based ion exchange media, which stands higher flow rate applications.

Sepax developed narrowly dispersed polymethacrylate-based ion exchange process media. The particle size of the resin is 30 μm with an average pore size of 1000 Å. The highly uniformly dispersed resin has a narrow particle distribution of D90/D10<1.3. The base resin is made of hydrophilic polymethacrylate. On the resin surface is covalently linked with different ion exchange functional groups, strong cation exchange group (sulfonic acid), strong anion exchange group (trimethyl quaternary amine) and weak anion exchange group (diethylamine).

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