Technical Support
(877) SEPAX-US, press 3 TechSupport@sepax-tech.com |
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Purification - HIC
Generik MC-HIC Butyl and Polar MC-HIC Butyl and Ether
Both Generik MC-HIC and Polar MC-HIC are made of polymethacrylate matrix with an average particle size of 30 µm and pore size of 800 Å, while Generik MC is more hydrophobic than Polar MC.
Hydrophobicity Order of three HIC 30 µm Resins:
Polar MC-HIC Ether < Polar MC-HIC Butyl < Generik MC-HIC Butyl
The resins can tolerate high-pressure, operation up to 100 bars, resulting in higher flow rate and shorter run time without compromising separation resolution.
The resins provide high recovery and resolution purification of mAb, ADC, Proteins, Oligo, and various biomolecules and are applicable for different stages of purification needs, from laboratory discovery, pilot-scale purification to industrial process chromatography.
Brand | Product | Matrix | Mean Particle Size / Pore Size |
Dynamic Binding Capacity |
Pressure Limit |
Max. Flow Rate |
---|---|---|---|---|---|---|
Sepax | Generik MC30-HIC Butyl |
Polymethacrylate | 30 µm / 800 Å |
40-50 mg / mL Lysozyme |
100 bar | 7200 cm/h |
Polar MC30-HIC Butyl |
30-40 mg / mL Lysozyme |
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Polar MC30-HIC Ether |
10-20 mg / mL Lysozyme |
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Tosoh | TSKGel Ether-5PW | Polymethacrylate | 30 µm / 1000 Å |
10-30 mg / mL Lysozyme |
20 bar | 1200 cm/h |
Toyopearl Butyl-650S |
35 µm / 1000 Å |
30-50 mg / mL Lysozyme |
3 bar | 600 cm/h | ||
GE | Butyl Sepharose High Performance |
Cross-linked agarose |
34 µm | 38 mg / mL ß-lactoglobulin |
2 bar | 150 cm/h |