Zenix™-C SEC phase, developed based on innovative surface coating technology, comprises of uniform, hydrophilic, and neutral nanometer thick film chemically bonded to high purity and mechanically stabilized silica. Zenix™-C, uses lay-down monolayer on porous silica as oppose to Zenix of stand-up monolayer (as shown below), offers ideal phase chemistry for hydrophobic samples like insulin, membrane protein monoclonal antibodies derivatized with polymer branches (polypeptide or PEG) separation. The 3µm based packing material allows for high resolution and performance separation.

Stationary Phase Structure Illustration and Comparison

Phase structure difference: a monolayer stands up on the silica surface for SRT® and Zenix™, and a monolayer lays down on the silica surface for SRT®-C and Zenix™-C.

Sepax SEC Product Family Comparison Chart

Product Line	SRT®	Zenix™	SRT®-C	Zenix™-C
Particle size	5 µm	3 µm	5 µm	3 µm
Pore size (Å)	100, 150, 300, 500, 1000 & 2000	80, 100, 150, 300	100, 150, 300, 500, 1000 & 2000	80, 100, 150, 300
Resolution	High	Highest, Short column for faster separation	High	Highest, Short column for faster separation
Efficiency	High	Doubled from 5µm	High	Doubled from 5µm
Selectivity	Same for SRT® and Zenix™		Same for SRT®-C and Zenix™-C
Surface structure	Chemically bonded stand-up monolayer		Chemically bonded lay-down monolayer
Recommended Sample Types	Monoclonal antibodies, proteins, peptides, nucleic acids, oligonucleotides, virus, and water-soluble polymers		"Tough samples" such as hydrophobic proteins like insulin, membrane protein monoclonal antibodies derivatized with polymer branches, e.g. polypeptide, PEG.

Zenix-C Technical Specifications

Phase	Zenix-C SEC-100	Zenix-C SEC-150	Zenix-C SEC-300
Material	Neutral, hydrophilic film bonded silica
Particle size	3 µm
Pore size	~ 100 Å	~ 150 Å	~ 300 Å
Protein MW range (native)	100 - 100,000	500 - 150,000	5,000 - 1,250,000
pH stability	2 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
Backpressure (for a 7.8×300 mm)	~ 1,500 psi	~ 1,375 psi	~ 1,100 psi
Backpressure (for a 4.6×300 mm)	~ 1,400 psi	~ 1,250 psi	~ 1,000 psi
Maximum backpressure	~ 4,500 psi	~ 4,500 psi	~ 3,500 psi
Salt concentration range	20 mM - 2.0 M
Maximum temperature	~ 80 °C
Mobile phase compatibility	Aqueous and organic

• Ideal for separation and analysis of hydrophobic proteins, and monoclonal antibodies derivatized with polymer branches such as polypeptide or PEG
• Particle size of 3 µm
• Selection of pore size: 100, 150 and 300 Å
• Highest separation efficiency and resolution
• High capacity
• High lot-to-lot reproducibility
• High protein recovery with intact biological activity
• Negligible non-specific interactions

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Separation of a protein mixture by Zenix-C SEC-300 column.

Conditions Columns: Zenix-C SEC-300(3 µm, 7.8×300mm) Mobile phase: 150 mM Sodium Phosphate, pH 7.0 Flow rate: 1.0 mL/min Temperature: Ambient (~23 °C) Detection: UV 214 nm Injection volume: 10 µL Samples: 1) Thyroglobulin, 670kD 2) γ-Globulin, 158 kD 3) Ovalbumin, 44kD 4) Myoglobin, 17.6 kD 5) Poly-DL-alanine (1-5 kD) 6) B12, 1.35 kD

Separation of Biorad protein mixture by Zenix-C SEC 100, 150 and 300 columns.

Conditions Columns: Zenix-C (3 µm, 7.8×300mm) Mobile phase: 150 mM Sodium Phosphate, pH 7.0 Flow rate: 1.0 mL/min Detection: UV 214 nm Injection volume: 10 µL Samples: 1) Thyroglobulin, 670kD 2) γ-Globulin, 158 kD 3) Ovalbumin, 44kD 4) Myoglobin, 17.6 kD 5) B12, 1.35 kD

Separation of protein mixture A by Zenix-C SEC 100, 150 and 300 columns.

Conditions Columns: Zenix-C (3 µm, 7.8×300mm) Mobile phase: 150 mM Sodium Phosphate, pH 7.0 Temperature: Ambient (~23 °C) Flow rate: 1.0 mL/min Detection: UV 214 nm Injection volume: 10 µL Samples: 1) Thyroglobulin, 670kD 2) BSA, 66kD 3) Ribonuclease A, 13.7kD 4) Uracil , 120D

Protein molecular weight vs elution volume is plotted in below figure, indicating Zenix-C 100, 150 and 300 have large linear elution region.

Protein MW calibration with elution volume for Zenix-C phases.

Conditions Columns: 3 µm, 7.8×300mm Mobile phase: 150 mM Sodium Phosphate, pH 7.0 Flow rate: 1.0 mL/min Detection: UV 214 nm Injection volume: 10 µL Samples: 1. Thyroglobulin, 670 kD 2. γ-Globulin, 158 kD 3. BSA, 66 kD 4. Ovalbumin, 44 kD 5. Myoglobin, 17.6 kD 6. Ribonuclease A, 13.7 kD 7. B12, 1.35 kD 8. Uracil, 120

Fusion Protein

Monoclonal Antibody

Oligonucleotide

Peptide

Protein

Zenix-C SEC-80    Zenix-C SEC-100    Zenix-C SEC-150    Zenix-C SEC-300

Sepax Zenix-C SEC-80 Sign In to see pricing Standard Dimension: Part Number Particle Size Pore Size ID×Length 233080-4605 3 µm 80 Å 4.6×50mm 233080-4630 3 µm 80 Å 4.6×300mm 233080-7805 3 µm 80 Å 7.8×50mm 233080-7830 3 µm 80 Å 7.8×300mm Coupler P/N: 102002-COUPLER SS with PEEK ferrule inserts, recommended to connect columns in series. price: $75/ea Sepax Zenix-C SEC-100 Sign In to see pricing Standard Dimension: Part Number Particle Size Pore Size ID×Length 233100-4605 3 µm 100 Å 4.6×50mm 233100P-4605 [3] 3 µm 100 Å 4.6×50mm 233100-4615 3 µm 100 Å 4.6×150mm 233100-4630 3 µm 100 Å 4.6×300mm 233100P-4630 [3] 3 µm 100 Å 4.6×300mm 233100-7805 3 µm 100 Å 7.8×50mm 233100-7815 3 µm 100 Å 7.8×150mm 233100-7830 3 µm 100 Å 7.8×300mm 233100-10005 3 µm 100 Å 10.0×50mm 233100-10015 3 µm 100 Å 10.0×150mm 233100-10030 3 µm 100 Å 10.0×300mm 233100-21205 3 µm 100 Å 21.2×50mm 233100-21230 3 µm 100 Å 21.2×300mm [3] Column packed with PEEK tubing. Coupler P/N: 102002-COUPLER SS with PEEK ferrule inserts, recommended to connect columns in series. price: $75/ea Sepax Zenix-C SEC-150 Sign In to see pricing Standard Dimension: Part Number Particle Size Pore Size ID×Length 233150-4605 3 µm 150 Å 4.6×50mm 233150P-4605 [3] 3 µm 150 Å 4.6×50mm 233150-4615 3 µm 150 Å 4.6×150mm 233150-4630 3 µm 150 Å 4.6×300mm 233150P-4630 [3] 3 µm 150 Å 4.6×300mm 233150-7805 3 µm 150 Å 7.8×50mm 233150-7815 3 µm 150 Å 7.8×150mm 233150-7830 3 µm 150 Å 7.8×300mm 233150-10005 3 µm 150 Å 10.0×50mm 233150-10015 3 µm 150 Å 10.0×150mm 233150-10030 3 µm 150 Å 10.0×300mm 233150-21205 3 µm 150 Å 21.2×50mm 233150-21230 3 µm 150 Å 21.2×300mm [3] Column packed with PEEK tubing. Coupler P/N: 102002-COUPLER SS with PEEK ferrule inserts, recommended to connect columns in series. price: $75/ea Sepax Zenix-C SEC-300 Sign In to see pricing Standard Dimension: Part Number Particle Size Pore Size ID×Length 233300-4605 3 µm 300 Å 4.6×50mm 233300P-4605 [3] 3 µm 300 Å 4.6×50mm 233300-4615 3 µm 300 Å 4.6×150mm 233300-4630 3 µm 300 Å 4.6×300mm 233300P-4630 [3] 3 µm 300 Å 4.6×300mm 233300-7805 3 µm 300 Å 7.8×50mm 233300-7815 3 µm 300 Å 7.8×150mm 233300-7820 3 µm 300 Å 7.8×200mm 233300-7830 3 µm 300 Å 7.8×300mm 233300-10005 3 µm 300 Å 10.0×50mm 233300-10015 3 µm 300 Å 10.0×150mm 233300-10030 3 µm 300 Å 10.0×300mm 233300-21205 3 µm 300 Å 21.2×50mm 233300-21230 3 µm 300 Å 21.2×300mm [3] Column packed with PEEK tubing. Coupler P/N: 102002-COUPLER SS with PEEK ferrule inserts, recommended to connect columns in series. price: $75/ea SEC Screening Kit: P/N Phase ID × Length Pore Size SECKIT-7830 Zenix 7.8 × 300 mm 300 Å Zenix-C 7.8 × 300 mm 300 Å SECKIT-4630 Zenix 4.6 × 300 mm 300 Å Zenix-C 4.6 × 300 mm 300 Å

Catalog

User Manual

SEC Screening Kit

Small Biomolecule Separation