Zenix™-C SEC phase, developed based on innovative surface coating technology, comprises of uniform, hydrophilic, and neutral nanometer thick film chemically bonded to high purity and mechanically stabilized silica. Zenix™-C, uses lay-down monolayer on porous silica as oppose to Zenix of stand-up monolayer (as shown below), offers ideal phase chemistry for hydrophobic samples like insulin, membrane protein monoclonal antibodies derivatized with polymer branches (polypeptide or PEG) separation. The 3µm based packing material allows for high resolution and performance separation.
Stationary Phase Structure Illustration and Comparison
Phase structure difference: a monolayer stands up on the silica surface for SRT®
and Zenix™, and a monolayer lays down on the silica surface for SRT®
-C and Zenix™-C.
Sepax SEC Product Family Comparison Chart
|Particle size||5 µm||3 µm||5 µm||3 µm|
|Pore size (Å)||100, 150, 300, 500, 1000 & 2000||80, 100, 150, 300||100, 150, 300, 500, 1000 & 2000||80, 100, 150, 300|
|Resolution||High||Highest, Short column for faster separation||High||Highest, Short column for faster separation|
|Efficiency||High||Doubled from 5µm||High||Doubled from 5µm|
|Selectivity||Same for SRT® and Zenix™||Same for SRT®-C and Zenix™-C|
|Surface structure||Chemically bonded stand-up monolayer||Chemically bonded lay-down monolayer|
|Recommended Sample Types||Monoclonal antibodies, proteins, peptides, nucleic acids, oligonucleotides, virus, and water-soluble polymers||"Tough samples" such as hydrophobic proteins like insulin, membrane protein monoclonal antibodies derivatized with polymer branches, e.g. polypeptide, PEG.|