Desalting RP columns are specifically designed for removing salts from aqueous biomolecule samples such as peptides, proteins and DNAs while enabling online MS analyses of the biomolecules. The packing support is composed of porous, spherical, and highly cross-linked poly(styrene/divinylbenzene) (PS/DVB) beads with a particle size of 5 μm. The column is compatible with most aqueous buffers, such as acetate, phosphate, tris, etc.
Sepax Desalting RP columns come in a combo pack containing 3 columns and one holder.
Packing
Highly cross-linked PS/DVB resin support grafted with a densely packed, nanometer thick amphiphilic coating.
Particle Size
5 µm
Pore Structure
Porous
pH Stability
2-13 (working pH)
Operating temperature limit
80 °C
Operating pressure limit
200 bar
Mobile phase compatibility
Compatible with aqueous buffer solutions: phosphate, tris and acetate, as well as a mixture of water and acetonitrile (ACN), isopropanol (IPA), acetone, or methanol.
Flow Rate
0.1-0.5 mL/min is typical for a 2.1 mm I.D. column
[1] Pack of three with one cartridge holder. [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series
