Sepax SRT-10 and SRT-10C SEC and Columns – FPLC* and HPLC Compatible
Description
Packing
Characteristics
Application
Order Info
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Utilizing proprietary surface technologies, SRT-10 and SRT-10C SEC phases are made of uniform, hydrophilic, and neutral nanometer thick films chemically bonded on high purity and mechanically stabilized 10 µm silica. The well-controlled surface chemistry results in excellent lot-to-lot reproducibility.
Our unique bonding chemistry, coupled with the maximized bonding density, allows SRT-10 and SRT-10C SEC phases to provide high stability and negligible non-specific interactions. SRT-10 and SRT-10C packings have large pore volume, resulting in high separation resolution.
The narrowly dispersed, spherical silica particles of the SRT-10 SEC-100, 300, 500 and 2000 have nominal pore sizes at 100 Å, 300 Å, 500 Å and 2000 Å, respectively.
Typical applications for SRT-10 and SRT-10C columns include separation and detection of biological molecules and water-soluble polymers in aqueous buffers.
SEC Surface Chemistry Advantage
Stand-up Monolayer
Lay-down Monolayer
Zenix (3 µm) SRT (5 µm) SRT-10 (10 µm)
Zenix-C (3 µm) SRT-C (5 µm) SRT-10C (10 µm)
Ideal for Non-hydrophobic samples:
Various types of non-hydrophobic protein including MAb and etc.
Ideal for Hydrophobic samples:
ADC, Derivatized MAb, Pegylated Protein, Membrane Protein, "Super sticky protein", and etc.
Fast High Resolution Purification on FPLC
Product
Sepax SRT/SRT-C SEC-300 21.2x300mm, 5µm
Sepax SRT-10/10C SEC-300 21.2x300mm, 10µm
GE Superdex 200 16x600mm, 34µm
Product Code
215300-21230/ 235300-21230
225300-21230/ 239300-21230
28-9893-35
Column Volume
105 mL
105 mL
120 mL
Flow Rate
7-10 mL/min
7-10 mL/min
0.75-1 mL/min
Run Time
< 20 min
< 20 min
> 2 Hour
Backpressure
55-70 bar
15-27 bar (well below the pressure limit of the AKTA FPLC system)
Superdex max column backpressure tolerance is 3 bar
Max Loading
5 mL
~42mg (BSA) / 5ml
5ml
Characteristics
Fast High Res Purification
Fast Purification
Cut your run time from 2 hour to 20 minutes (85% of your time saved)
with same or higher resolution delivered
SRT-10/10C Technical Specifications
Phase
SRT-10/10C SEC-100
SRT-10/10C SEC-300
SRT-10/10C SEC-500
SRT-10/10C SEC-1000
Material
Neutral, hydrophilic film bonded silica
Particle size
10 µm
10 µm
10 µm
10 µm
Pore size (Å)
~ 100
~ 300
~ 500
~ 1000
Protein MW range (native)
100 - 30,000
5,000 - 1,250,000
15,000 - 5,000,000
50,000 - 7,500,000
pH stability
2.5 – 7.5 (up to pH 8.5 can be tolerated temporarily*)
Backpressure (7.8×300 mm)
~ 300 psi (flow rate at 1.0 mL/min)
Maximum backpressure (psi)
~ 3,000
Salt concentration range
20 mM – 500 mM
Maximum temperature (°C)
~4 – 40°C
Mobile phase compatibility
Aqueous and organic
Sample Loading Recommendation
ID
4.6×300 mm
7.8×300 mm
10×300mm
21.2×300mm
30×300mm
50×300mm
Column Volume
5 mL
14 mL
24 mL
105 mL
212 mL
490 mL
V-injection
5-100 µL
15-250 µL
25-940 µL
0.1-4.2 mL
0.2-8.5 mL
0.5-20 mL
Column Loading Guideline (BSA)
<= 1 mg
<= 2.5 mg
<= 9 mg
<= 42 mg
<= 85 mg
<= 235 mg
Standard Flow rate (Maximum)
0.35 mL/min
1.0 mL/min
1.65 mL/min (2.0 mL/min)
7.5 mL/min (10mL/min)
15 ml/min (25 ml/min)
41 ml/min (60 ml/min)
Typical Run Time
< 20 min
< 20 min
< 20 min
< 20 min
< 20 min
< 20 min
Back pressure
< 400 psi < 30 bar < 3 MPa
< 700 psi < 50 bar < 5 MPa
< 900 psi < 60 bar < 6 MPa
< 900 psi < 60 bar < 6 MPa
< 900 psi < 60 bar < 6 MPa
< 900 psi < 60 bar < 6 MPa
Particle size: 10 µm
Pore size selection: 100, 300 500 and 2000 Å
pH stability 2-8.5
High capacity and resolution
High stability over low and high concentration salt
Excellent lot-to-lot reproducibility
High protein recovery with intact biological activity
Chemically bonded stationary phase resulting in negligible non-specific interactions
Ideal for separation of biological molecules: proteins, nucleic acids, oligonucleotides, peptides and virus
Ideal for separation of natural polymers, e.g. polysaccharides, water soluble synthetic polymers, and nanomaterials, e.g. nanoparticles
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [6] Intended for use with column IDs ≥ 21.2 mm [7] For Precolumn Filter (2 µm) 5 units/pk [8] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [6] Intended for use with column IDs ≥ 21.2 mm [7] For Precolumn Filter (2 µm) 5 units/pk [8] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [6] Intended for use with column IDs ≥ 21.2 mm [7] For Precolumn Filter (2 µm) 5 units/pk [8] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [6] Intended for use with column IDs ≥ 21.2 mm [7] For Precolumn Filter (2 µm) 5 units/pk [8] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [6] For Precolumn Filter (2 µm) 5 units/pk
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [6] Intended for use with column IDs ≥ 21.2 mm [7] For Precolumn Filter (2 µm) 5 units/pk [8] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [6] Intended for use with column IDs ≥ 21.2 mm [7] For Precolumn Filter (2 µm) 5 units/pk [8] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [6] Intended for use with column IDs ≥ 21.2 mm [7] For Precolumn Filter (2 µm) 5 units/pk [8] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [6] Intended for use with column IDs ≥ 21.2 mm [7] For Precolumn Filter (2 µm) 5 units/pk [8] SS Refill Frits for 102020-21200, 5 units/pk
[1] One-piece PEEK coupler, recommended to connect columns in series. [2] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [3] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series
