Utilizing proprietary innovative surface technologies, Unix™-C SEC-300 phase is made of uniform, hydrophilic, and neutral nanometer thick films chemically bonded to high purity and mechanically stabilized silica with the particle size of 1.8 µm. The combination of small particle size and large pore volume of Unix™-C SEC-300 renders the highest separation efficiency and resolution of analytes. The well-controlled surface chemistry results in excellent lot-to-lot reproducibility. The unique bonding chemistry, coupled with the maximized bonding density, allows Unix™-C SEC-300 to provide high stability and negligible non-specific interactions.
Unix™-C, uses lay-down monolayer on porous silica (as shown below), offers ideal phase chemistry for the separation of all biological sample types including challenging hydrophobic samples such as antibody drug conjugates (ADCs), hydrophobic mAb and its fragments, bispecific mAbs (BsMAbs), PEGylated proteins, glycoproteins, fusion proteins, membrane proteins and oligonucleotides.
Unix-C SEC Technical Specifications
Phase
UnixTM-C SEC-300
Material
Neutral, hydrophilic film bonded silica
Average Particle size
1.8 µm
Pore size
300 Å
Protein MW range (native)
5,000 - 1,250,000 Da
pH stability1
2.0 - 8.5
Mobile phase compatibility
Aqueous and organic
Suggested salt concentration
< 0.5 M
Recommended flow rate range for maximum column lifetime
0.1 - 0.35 mL/min
Recommended operating column backpressure for maximum column lifetime
< 4,500 psi
Suggested operating temperature for optimal column lifetime
10 - 30°C
1 Store the column in neutral pH
Particle size of 1.8 µm with a pore size of 300 Å
Ultra-high separation efficiency, recovery, and resolution
High stability and lot-to-lot reproducibility
Innovative surface chemistry for minimized secondary interactions
Reduced dependency on organic additives for hydrophobic samples
Ideal for all sample types including challenging hydrophobic samples like ADCs, bsMAbs, PEGylated proteins, glycoproteins, fusion proteins, membrane proteins, and oligonucleotides.
Great tool for universal platform high throughput method screening (HTS)
[1] Guard column [2] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [3] One-piece PEEK coupler, recommended to connect columns in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 0.5 µm Stainless Steel frit for column with particle size 3 µm or less [6] For Precolumn Filter (0.5 µm) 5 units/pk [7] Sepax, Precolumn Filter kit to be used with UHPLC columns. Includes (1) PEEK Precolumn Filter with 0.5 um stainless steel frit (102000-P346) and (1) PEEK coupler (102002-COUPLER)
