Utilizing proprietary surface technologies, Unix SEC-300 phase is made of uniform, hydrophilic, and neutral nanometer thick films chemically bonded to high purity and mechanically stabilized silica with the particle size of 1.8 µm. The combination of small particle size and large pore volume of Unix SEC-300 renders the highest separation efficiency and resolution of analytes. The well-controlled surface chemistry results in excellent lot-to-lot reproducibility. Our unique bonding chemistry, coupled with the maximized bonding density, allows Unix SEC-300 to provide high stability and negligible non-specific interactions. Typical applications for Unix SEC-300 columns include separation and analysis of biological molecules and water-soluble polymers.
Unix SEC Technical Specifications
Phase
UnixTM SEC-300
Material
Neutral, hydrophilic film bonded silica
Average Particle size
1.8 µm
Pore size
300 Å
Protein MW range (native)
5,000 - 1,250,000 Da
pH stability
2.0 – 7.6 (up to pH 8.5 can be tolerated temporarily*)
Excellent biomolecule separation while maintaining its original conformation due to low pressure resin property (up to 50% lower than competitor)
>50% faster separation than traditional SEC columns without compromising resolution for high throughput screening
Ideal for separation and analysis of biological molecules such as mAb, ADC, proteins, protein conjugates, and oligonucleotides
Ideal for separation and analysis of natural and synthetic polymers, and nanomaterials
High Separation Efficiency
The advantages of small particle size are higher efficiency and higher resolution. Column efficiency nearly doubles upon decreasing the particle size from 5 µm to 1.8 µm (Figure 1 and 2).
Figure 1. Comparison of Unix™ 1.8 µm, Zenix® 3 µm, and SRT® 5 µm SEC-300 on Protein Standards
Column:
Unix™ SEC-300 (1.8 µm, 300 Å, 4.6 x 150 mm)
Zenix® SEC-300 (3 µm, 300 Å, 4.6 x 150 mm)
SRT® SEC-300 (5 µm, 300 Å, 4.6 x 150 mm)
Application of proprietary surface modification techniques to Unix™ SEC-300 resin produces a densely bonded silica surface, which greatly hinders the diffusion of molecules that would attack the bond between silica surface and tethered moieties, thus enabling high stability over a wide range of pH from 2.0 to 8.5.
Unix™ SEC-300 phase is compatible with most aqueous buffers, such as ammonium acetate, phosphate, trizma and so on. Unix™ SEC-300 phase can tolerate high concentration of salts. Furthermore, Unix™ SEC columns are stable in both organic solvents, such as methanol, ethanol, THF, DMF, DMSO, etc. as well as mixture of water and organic solvents.
Lot-to-Lot Reproducibility
The controlled surface chemistry used to synthesize Unix™ SEC-300 phase makes the surface coating highly reproducible, leading to consistent column manufacturing. Separation variation from batch-to-batch is controlled to be within 5% for retention time. Figure 5 demonstrates the lot-to-lot variation of three different Unix™ SEC-300 lots.
Figure 5. Unix™ Lot-to-Lot Reproducibility on Protein Standards
[1] Guard column [2] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [3] One-piece PEEK coupler, recommended to connect columns in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 0.5 µm Stainless Steel frit for column with particle size 3 µm or less [6] For Precolumn Filter (0.5 µm) 5 units/pk [7] Sepax, Precolumn Filter kit to be used with UHPLC columns. Includes (1) PEEK Precolumn Filter with 0.5 um stainless steel frit (102000-P346) and (1) PEEK coupler (102002-COUPLER)
[1] Guard column [2] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [3] One-piece PEEK coupler, recommended to connect columns in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 0.5 µm Stainless Steel frit for column with particle size 3 µm or less [6] For Precolumn Filter (0.5 µm) 5 units/pk [7] Sepax, Precolumn Filter kit to be used with UHPLC columns. Includes (1) PEEK Precolumn Filter with 0.5 um stainless steel frit (102000-P346) and (1) PEEK coupler (102002-COUPLER)
