SRT®-C SEC phase, developed based on innovative surface coating technology, comprises of uniform, hydrophilic, and neutral nanometer thick film chemically bonded to high purity and mechanically stabilized silica. SRT®-C, uses lay-down monolayer on porous silica as oppose to SRT of stand-up monolayer (as shown below), offers ideal phase chemistry for hydrophobic samples like insulin, membrane protein monoclonal antibodies derivatized with polymer branches (polypeptide or PEG) separation.
Stationary Phase Structure Illustration and Comparison
Phase structure difference: a monolayer stands up on the silica surface for SRT® and Zenix™, and a monolayer lays down on the silica surface for SRT®-C and Zenix™-C.
"Tough samples" such as hydrophobic proteins like insulin, membrane protein monoclonal antibodies derivatized with polymer branches, e.g. polypeptide, PEG.
SRT®-C Technical Specifications
Phase
SRT®-C SEC-100
SRT®-C SEC-150
SRT®-C SEC-300
SRT®-C SEC-500
SRT®-C SEC-1000
SRT®-C SEC-2000
Material
Neutral, hydrophilic film bonded silica
Particle size
5 µm
Pore size
~ 100 Å
~ 150 Å
~ 300 Å
~ 500 Å
~ 1000 Å
~ 2000 Å
Protein MW range (native) [Da]
100 - 100,000
500 - 150,000
5,000 - 1,250,000
15,000 - 5,000,000
50,000 - 7,500,000
> 10,000,000
pH stability
2.5 – 7.5 (up to pH 8.5 can be tolerated temporarily*)
Backpressure
~ 700 psi (for a 7.8×300 mm)
Maximum backpressure [psi]
~ 4,500
~ 4,500
~ 3,500
~ 3,000
~ 3,000
~ 3,000
Salt concentration range
20 mM – 500 mM
Maximum temperature
~4 – 40°C
Mobile phase compatibility
Aqueous and organic
Ideal for separation and analysis of hydrophobic proteins, and monoclonal antibodies derivatized with polymer branches such as polypeptide or PEG
Particle size of 5 µm
Selection of pore size: 100, 150, 300, 500, 1,000, and 2,000 Å
High separation efficiency and resolution
High capacity
High lot-to-lot reproducibility
High protein recovery with intact biological activity
Negligible non-specific interactions
Separation of a protein mixture by SRT-C SEC-300 column.
[1] Guard column [2] Column packed with PEEK tubing. [3] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [8] Intended for use with column IDs ≥ 21.2 mm [9] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] Column packed with PEEK tubing. [3] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [8] Intended for use with column IDs ≥ 21.2 mm [9] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] Column packed with PEEK tubing. [3] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [8] Intended for use with column IDs ≥ 21.2 mm [9] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] Column packed with PEEK tubing. [3] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [8] Intended for use with column IDs ≥ 21.2 mm [9] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] Column packed with PEEK tubing. [3] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [8] Intended for use with column IDs ≥ 21.2 mm [9] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] Column packed with PEEK tubing. [3] Sepax Protein standard for size exclusion chromatography and used for general correlation of elution time vs. protein MW [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [8] Intended for use with column IDs ≥ 21.2 mm [9] SS Refill Frits for 102020-21200, 5 units/pk
