Analytical and Preparative Size Exclusion
are eight SEC product lines developed based on innovative surface coating and particle technologies comprised of uniform, hydrophilic, and neutral nanometer thick films chemically bonded on 1.8 µm, 3 µm, 5 µm, or 10 µm high purity and mechanically stabilized silica.
Utilizing two unique surface coating technologies - stand-up monolayer bonded on porous silica for Unix™, Zenix®, SRT® and SRT®-10; and lay-down monolayer on porous silica for Zenix®-C, SRT®-C, and SRT®-10C, Sepax offers surface chemistry choices based on sample type and sample hydrophobicity in order to achieve high resolution performance separation.
Find Your Right Particle Size
|Particle size||1.8 µm||3 µm||5 µm||10 µm|
|Pore size (Å)||200, 300||80, 100, 150, 300||100, 150, 300, 500, |
|100, 300, 500, 1000|
|Resolution & |
|Instruments||UHPLC, HPLC||HPLC, UHPLC||HPLC, FPLC||HPLC, FPLC|
Find Your Suitable Surface Chemistry
||Unix™, Zenix®, SRT®, SRT®-10
||Zenix®-C, SRT®-C, SRT®-10C
||Chemically bonded stand-up monolayer
||Chemically bonded lay-down monolayer
|Recommended Sample Types
||Non-Hydrophobic samples like Monoclonal antibodies, proteins, peptides, nucleic acids, oligonucleotides, virus, and water-soluble polymers
||"Tough samples" such as Hydrophobic proteins like insulin, membrane protein monoclonal antibodies derivatized with polymer branches, e.g. polypeptide, PEG.
Nanofilm® is made with a special stationary phase - a polymeric brush coating. Such a neutral, hydrophilic polymer coating is suitable for positively charged proteins and hydrophobic proteins, such as membrane proteins.
|Particle size||5 µm|
|Pore size (Å)||150, 250, 450|
|Selectivity||Same for SRT® and Zenix™|
|Surface structure||Chemically bonded polymeric coating|
|Recommended Sample Types||Highly positively charged proteins, hydrophobic proteins like membrane protein|