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Technical Support
(877) SEPAX-US, press 3  TechSupport@sepax-tech.com |
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Proteomix HIC Phase Screening Kit
| P/N | Description | Buy or Quote |
|---|---|---|
| HICKIT-4603 | Proteomix HICButyl-NP5, 4.6×35 mm | |
| Proteomix HICPhenyl-NP5, 4.6×35 mm | ||
| Proteomix HICEthyl-NP5, 4.6×35 mm | ||
| Proteomix HICPropyl-NP5, 4.6×35 mm |
Proteomix® HIC Ethyl, HIC Propyl, HIC Butyl and HIC Phenyl phases are comprised of rigid, spherical, highly cross-linked non-porous polystyrene/divinylbenzene (PS/DVB) beads with narrow-dispersed particle distribution. Utilizing proprietary technologies, the resin surfaces are grafted with ethyl propyl, butyl or phenyl group that offers different degree of hydrophobicity, to facilitate chromatographic separation of biomolecules (Figure below).
Hydrophobicity is increased from ethyl, propyl, butyl to phenyl phase respectively, as shown in a separation of ovalbumin and chymotrysinogen under HIC running conditions.
| Conditions | |
| Columns: | 4.6 × 35 mm |
| Mobile phase: | A: 2 M ammonium sulfate in 0.1 M sodium phosphate, pH 7.0 B: 0.1 M phosphate pH 7.0 |
| Flow rate: | 0.4 mL/min |
| Temperature: | 25 °C |
| Detector: | UV 214 nm |
| Injection volume: | 4 µL |
| Sample: | Ovalbumin 1.0 mg/mL, Chymotrypsinogen 0.5 mg/mL |