Hydrophobic Interaction Chromatographic (HIC) separation applies to all stages of biological sample purification process including capturing and polishing. Sepax manufactures two HIC products: Generik MC-HIC and Polar MC-HIC. Both Polar MC-HIC and Generik MC-HIC are made of polymethacrylate matrix, while Generik MC is more hydrophobic than Polar MC.
Polar MC30-HIC chromatographic media is composed of hydrophilic polymethacrylate beads functionalized with ether or butyl on the surface, as shown in Figure 2, which provide appropriate hydrophobic characteristics and interactions with biological analytes. Those media have an average particle size of 30 µm and pore size of 800 Å. It has high physical and chemical stability and can tolerate high-pressure operation up to 100 bars which allows faster flow rate and shorter running time. These resins are specially designed for various stages of hydrophobic interaction chromatography separation needs, from laboratory discovery, pilot-scale purification to industrial process.
Polar MC30-HIC Butyl Polar MC30-HIC Ether
70% (v/v) slurry in 20% ethanol
20 - 45 µm (average 30 µm)
Dynamic Binding Capacity*
15 ± 5 mg Lysozyme /mL for Polar MC-HIC Ether
35± 5 mg Lysozyme /mL for Polar MC-HIC Butyl
Up to 40 °C
Up to 100 bar
Mobile Phase Compatibility
Compatible with aqueous solution, a mixture of water and acetonitrile, acetone, or methanol. Typical buffers: phosphate, Tris, & acetate.
Linear Flow Rate
Up to 7200 cm/hour
*DBC was tested with lysozyme (1 mg/mL) in 25 mM sodium phosphate pH 7 + 2 M (NH4)2SO4 with 360 cm/h flow rate at 280 nm, 10% breakthrough.
Unique hydrophobic interaction
High accessible surface area and loading capacity
High chemical stability for low leaching
High physical stability for high-pressure operation
Capable of running high flow rate to speed up separation
Spherical particles with high mechanical strength
Wide pH tolerance (pH=2-14)
Available from 1 L to >100 L
Polar MC30-HIC media offers excellent high efficiency and recovery separation of bio-molecules such as mAb (monoclonal antibody), ADC (antibody drug conjugate) and related protein fragments, DNA and oligonucleotides.