Description Packing Characteristics Application Order Info Downloads
Polar MC chromatographic resins are composed of hydrophilic polymethacrylate beads with high physical and chemical stability. The Polar MC resins have particle size of 30 and 60 µm with pore size of 800 Å. The resin surface is highly hydrophilic, which minimizes non-specific bindings with biological analytes, so resins could be used as without functional groups for size exclusion separations, or derivatized with different surface chemical groups for alternative modes of chromatography such as ion exchange, hydrophobic interaction or affinity separations.
Polar MC30/60-SCX, WCX, SAX and WAX resins are designed for ion exchange chromatography. On the surface of the hydrophilic polymethacrylate beads, ion-exchange functional groups are attached via chemical bonding. A proprietary chemistry was developed to synthesize a densely packed and uniform ion-exchange layer. These resins provide excellent separations for bio-molecules with high efficiency and recovery, and also tolerate high-pressure operation up to 100 bars.
Specification
Resins Type Polar MC30-SCX, WCX, SAX, WAX Polar MC60-SCX, WCX, SAX, WAX
Packing 70% (v/v) slurry in 20% ethanol
Matrix Hydrophilic polymethacrylate
Particle Size Polar MC30: 20 ~ 45 µm (mean 30 µm) Polar MC60: 45 ~ 90 µm (mean 60 µm)
Pore Structure 800 Å
Dynamic Binding Capacity* Polar MC30 SCX: 40 mg Lys./mL WCX: 35 mg Lys./mL SAX: 40 mg BSA/mL WAX: 35 mg BSA/mL
Polar MC60 SCX: 35 mg Lys./mL WCX: 20 mg Lys./mL SAX: 35 mg BSA/mL WAX: 20 mg BSA/mL
pH Stability 2-12
Ionic Capacity Polar MC30 SCX: 0.12 meq. /mL WCX: 0.12 meq. /mL SAX: 0.12 meq. /mL WAX: 0.09 meq. /mL
Polar MC60 SCX: 0.10 meq. /mL WCX: 0.10 meq. /mL SAX: 0.10 meq. /mL WAX: 0.09 meq. /mL
Operating Temperature Up to 40 °C
Operating Pressure Up to 100 bar
Mobile Phase Compatibility Compatible with aqueous solution, a mixture of water and acetonitrile, acetone, or methanol. Typical buffers: phosphate, Tris, & acetate.
Linear Flow Rate Up to 1800 cm/hour
* Polar MC SAX & WAX: 100 cm/hour, 50 mM Tris pH8.5, 2 mg/mL BSA;
Polar MC SCX & WCX: 360 cm/hour, 50 mM Sodium phosphate pH6.0, 1 mg/mL Lysozyme
High chemical stability for low leaching
Spherical particles with narrow particle size distribution
High accessible surface area and high loading capacity
High retentativity and selectivity
Wide pH applications (pH=2-13)
High lot to lot consistency
Available from grams to multi-Kilogram
High pressure limit
Purification of proteins, peptides, proteins, nucleic acids, oligonucleotides and antibodies
Suitable for heparin purification
Solid phase extraction
Flash chromatography
Process purification
Polar MC IEX Columns
Polar MC30 Polar MC60
Sepax Polar MC30Sign In to see pricing
Description Part Number Tubing Unit x Volume Dimension Buy or Quote
Polar MC30 4 IEX Phases Screening Kit 289030-75100 Plastic 4 x 4.2mL 7.5 x 100 mm Add Polar MC30-SAX 285030-6615 Glass 1x4.1mL 6.6×150mm Add
285030-7810 Stainless Steel 1x5mL 7.8×100mm Add
285030-15015 Glass 1x21.2mL 15.0×150mm Add
285030-21210 Stainless Steel 1x35mL 21.2×100mm Add
285030-750100 Plastic 1x4.2mL 7.5×100mm Add
Polar MC30-SCX 283030-6615 Glass 1x4.1mL 6.6×150mm Add
283030-7810 Stainless Steel 1x5mL 7.8×100mm Add
283030-15015 Glass 1x21.2mL 15.0×150mm Add
283030-21210 Stainless Steel 1x35mL 21.2×100mm Add
283030-750100 Plastic 1x4.2mL 7.5×100mm Add
Polar MC30-WAX 286030-6615 Glass 1x4.1mL 6.6×150mm Add
286030-7810 Stainless Steel 1x5mL 7.8×100mm Add
286030-15015 Glass 1x21.2mL 15.0×150mm Add
286030-21210 Stainless Steel 1x35mL 21.2×100mm Add
286030-750100 Plastic 1x4.2mL 7.5×100mm Add
Polar MC30-WCX 284030-6615 Glass 1x4.1mL 6.6×150mm Add
284030-7810 Stainless Steel 1x5mL 7.8×100mm Add
284030-15015 Glass 1x21.2mL 15.0×150mm Add
284030-21210 Stainless Steel 1x35mL 21.2×100mm Add
284030-750100 Plastic 1x4.2mL 7.5×100mm Add
Sepax Polar MC60Sign In to see pricing
Description Part Number Tubing Unit x Volume Dimension Buy or Quote
Polar MC60 4 IEX Phases Screening Kit 289060-75100 Plastic 4 x 4.2mL 7.5 x 100 mm Add Polar MC60-SAX 285060-6615 Glass 1x4.1mL 6.6×150mm Add
285060-7810 Stainless Steel 1x5mL 7.8×100mm Add
285060-15015 Glass 1x21.2mL 15.0×150mm Add
285060-21210 Stainless Steel 1x35mL 21.2×100mm Add
285060-750100 Plastic 1x4.2mL 7.5×100mm Add
Polar MC60-SCX 283060-6615 Glass 1x4.1mL 6.6×150mm Add
283060-7810 Stainless Steel 1x5mL 7.8×100mm Add
283060-15015 Glass 1x21.2mL 15.0×150mm Add
283060-21210 Stainless Steel 1x35mL 21.2×100mm Add
283060-750100 Plastic 1x4.2mL 7.5×100mm Add
Polar MC60-WAX 286060-6615 Glass 1x4.1mL 6.6×150mm Add
286060-7810 Stainless Steel 1x5mL 7.8×100mm Add
286060-15015 Glass 1x21.2mL 15.0×150mm Add
286060-21210 Stainless Steel 1x35mL 21.2×100mm Add
286060-750100 Plastic 1x4.2mL 7.5×100mm Add
Polar MC60-WCX 284060-6615 Glass 1x4.1mL 6.6×150mm Add
284060-7810 Stainless Steel 1x5mL 7.8×100mm Add
284060-15015 Glass 1x21.2mL 15.0×150mm Add
284060-21210 Stainless Steel 1x35mL 21.2×100mm Add
284060-750100 Plastic 1x4.2mL 7.5×100mm Add
Polar MC IEX Bulk Resin
Phase Particle Size Pore Size P/N
Polar MC30-SCX 30 µm 800 Å 283030-0000
Polar MC30-WCX 30 µm 800 Å 284030-0000
Polar MC30-SAX 30 µm 800 Å 285030-0000
Polar MC30-WAX 30 µm 800 Å 286030-0000
Polar MC60-SCX 60 µm 800 Å 283060-0000
Polar MC60-WCX 60 µm 800 Å 284060-0000
Polar MC60-SAX 60 µm 800 Å 285060-0000
Polar MC60-WAX 60 µm 800 Å 286060-0000
Package size: 10mL, 100mL, 250mL, 500mL, 1L, 10L, 50L and others for request.
The resins are stored and shipped in 20% ethanol in water. Dry resin is available upon request.
Related Product- GenerikĀ® FPLC Empty Column
Part Number
End Fitting
Column Size (mm)
Bed Height (cm)
Volume (mL)
Quote
202000-0615-AF
A/F
6.6x150
4-12
1.4-4.1
Add
202000-0615-FF
F/F
6.6x150
12
4.1
Add
202000-0625-AA
A/A
6.6x250
6-22
2.1-7.5
Add
202000-0625-AF
A/F
6.6x250
14-22
4.8-7.5
Add
202000-0640-AA
A/A
6.6x400
21-37
7.2-12.7
Add
202000-1015-AF
A/F
10.0x150
4-12
3.1-9.4
Add
202000-1025-AA
A/A
10.0x250
6-22
3.1-17.3
Add
202000-1025-AF
A/F
10.0x250
14-22
19.63
Add
202000-1515-AF
A/F
15.0x150
4-12
7.1-21.2
Add
202000-1525-AA
A/A
15.0x250
6-22
10.6-38.9
Add
202000-1525-AF
A/F
15.0x250
14-22
24.7-38.9
Add
202000-2515-AF
A/F
25.0x150
4-12
19.6-58.9
Add
202000-2525-AA
A/A
25.0x250
6-22
29.4-108.0
Add
202000-2525-AF
A/F
25.0x250
14-22
68.7-108.0
Add
202000-3540-AF
A/F
35.0x400
29-37
279.0-355.9
Add
202000-5015-AF
A/F
50.0x150
4-12
77.5-235.0
Add
202000-5025-AA
A/A
50.0x250
5.8-22.0
120.2-440.7
Add
202000-5040-AA
A/A
50.0x400
21-37
420.5-741.0
Add
202000-5040-AF
A/F
50.0x400
29-37
580.7-741.0
Add
202000-5050-AA
A/A
50.0x500
31-47
620.8-941.2
Add
202000-5050-AF
A/F
50.0x500
39-47
781.0-941.2
Add
AF: One fixed endpiece and one adjustable endpiece
AA: Two adjustable endpieces
FF: Non-adjustable with two fixed endpieces