HP-C18 uses full coverage bonded silica packing, which provides high stability. Compatible with 100% aqueous mobile phase and suitable for separations of acidic, neutral and basic organic compounds, as well as pharmaceuticals and peptides. ODS monolayer formed by special bonding chemistry does not collapse in pure aqueous solution.
Silica:
Spherical, high purity (<10 ppm metals)
Pore size:
120 Å
Particle size:
3, 5, and 10 µm
Pore volume:
1.0 ml/g
Surface area:
300 m²/g
Phase structure:
Monomeric and fully endcapped
% Carbon:
17%
Silica:
Spherical, high purity (<10 ppm metals)
Pore size:
200 Å
Particle size:
3 and 5 µm
Pore volume:
1.0 ml/g
Surface area:
200 m²/g
Phase structure:
Monomeric and fully endcapped
% Carbon:
10%
Highly controlled chemistry of monolayer formation and end-capping
Extremely high column-to-column reproducibility
High selectivity and efficiency for separations
Compatible with 100% aqueous mobile phase
Suitable for separations of acidic, neutral and basic organic compounds, as well as pharmaceuticals, peptides, and others
Stability
Sepax HP-C18 uses full coverage bonded silica packing, which provides the exceptionally high stability. Figure 1 shows the highly reproducible retention time for three standard compounds: aniline, anisole and toluene after 13,000 column volume runs in a mobile phase of 85% methanol and 15% water. Such high stability allows Sepax HP-C18 extremely suitable for validation of various analytes.
Reproducibility
Figure shown below is a real application of Sepax HP-C18, showing fast separation of 8 pharmaceutical molecules by a 3 µm, 4.6×50mm short column. After 1800 column volume of "0.1% TFA and CH3CN mobile phase" was pumped, the column still shows almost identical performance!
(Courtesy of Miyako Kawakatsu, M&S Instruments Inc.)
Selectivity
Sepax HP-C18 has better selectivity and resolution for organometallic complex isomers compared to other competitors' C18 phases.
(Courtesy of Dr. John Lin of Avantix Laboratories)
Conditions
Column selection:
1. Phenomenex, Synergi, Polar-RP, 4 µm, 50×2.0 mm
2. Keystone, AQ C18, 3 µm, 50×2.0 mm
3. MetaChem, Polaris-C18, 3 µm, 50×2.1 mm
4. Sepax HP C18, 3 µm, 50×2.1 mm
Mobile phase:
A: 0.1% TFA in water with 10 mM ammonium formate
B: 0.1% TFA in methanol with 10 mM ammonium formate
Gradient elution
Flow rate:
0.5 mL/min
Injection volume:
10 µL
Temperature:
25 °C
Instrument:
API 4000 LC-MS/MS system
Compounds:
Two organometallic complex isomers: M40403 and M40531
(The molecular structures were not disclosed from the customer.)
[1] Guard column [2] Guard Cartridge only. [3] Guard Cartridge with holder. [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] 0.5 µm PEEK frit for column with particle size 3 µm or less [8] For Precolumn Filter (0.5 µm) 5 units/pk
[1] Guard column [2] Guard Cartridge only. [3] Guard Cartridge with holder. [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] 0.5 µm PEEK frit for column with particle size 3 µm or less [8] For Precolumn Filter (0.5 µm) 5 units/pk
[1] Guard column [2] Guard Cartridge only. [3] Guard Cartridge with holder. [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [8] Intended for use with column IDs ≥ 21.2 mm [9] For Precolumn Filter (2 µm) 5 units/pk [10] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] Guard Cartridge only. [3] Guard Cartridge with holder. [4] One-piece PEEK coupler, recommended to connect columns in series. [5] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [6] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [7] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [8] Intended for use with column IDs ≥ 21.2 mm [9] For Precolumn Filter (2 µm) 5 units/pk [10] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [6] Intended for use with column IDs ≥ 21.2 mm [7] For Precolumn Filter (2 µm) 5 units/pk [8] SS Refill Frits for 102020-21200, 5 units/pk
[1] Guard column [2] One-piece PEEK coupler, recommended to connect columns in series. [3] PEEK/Stainless Steel coupler, recommended to connect columns of IDs > 7.8 mm in series. [4] Column coupler with 10cm length flexible tubing,10-32 fitting with 1/16 OD PEEK tubing. Recommended to connect analytical columns in series [5] 2 µm PEEK frit for column with particle size 5 µm and 10 µm. [6] Intended for use with column IDs ≥ 21.2 mm [7] For Precolumn Filter (2 µm) 5 units/pk [8] SS Refill Frits for 102020-21200, 5 units/pk
