ProAqa Excel Protein A Affinity HPLC column is specifically designed for fast, accurate and robust titer determination and quantification of monoclonal antibody during cell line selection or upstream bioprocess optimization and quality control.
The base resin is composed of monosized 20 µm 1000-2000 Å highly crossed linked PS/DVB beads coupled with a recombinant Protein A ligand which binds Fc-containing immunoglobin protein except IgG3. The resin is mechanically robust and can tolerate high-flow and high-pressure operation.
Rapid Antibody Titer Determination
Up to 3 mL/min
0.5 minutes total cycle time, including equilibration
Wide linear response range up to 40 mg/mL:
UV 280 nm: 0.029 - 7.50 mg/mL, with LOD 0.294 µg
UV 300 nm: 0.117 - 40.0 mg/mL
Robust: > 2000 Injection Lifetime
Reproducible: High Lot to Lot Consistency
Applicable with various amount of CHO cell lysate proteins
Compatible with UPLC, HPLC and FPLC systems
ProAqa Excel Base Resin SEM
Monosized beads (20 µm) with D90/D10<1.3