Proteomix POR50-dT20

Sepax Proteomix POR50-dT20 Affinity Resin is a monodispersed PS/DVB base bead that has a profuse porous structure with large through pores, good physical and chemical stability and good pressure resistance. Oligo dT20-mer is conjugated to the bead surface through a proprietary method. The resulting affinity resin is specially designed and highly optimized for the isolation of messenger RNA (mRNA).

Most mRNA molecules contain a tail of poly(adenylic acid) (polyA tail), up to 250 bases in length. The Proteomix POR dT20 Affinity Resin surface allows capture of mRNA through base pairing between oligo dT20-mer and the mRNA polyA tail using a simple and convenient chromatography procedure.

Features

• Provides efficient capture and release under standard mRNA purification conditions, simplifies subsequent purification steps, and maximizes overall production efficiency
• Large pore sizes which enables high loading capacity for large mRNAs, easier elution, and high recovery rate.
• Decreases process development time and enhances productivity.
• Allows reduction in plasmid DNA and other transcription mix components.
• Stable at elevated temperatures for the breakdown of undesired higher order structures, if needed.
• Excellent scalability. Available as prepacked columns, semi-prep columns, prep columns, and bulk resin.
• Non-animal derived

Technical Specifications

Bulk Resin

Proteomix POR50-dT20, Pre-Packed Column (7.5 x 100 mm, 4.2mL)

Proteomix POR50-dT20, Pre-Packed HPLC Column (4.6 x 50 mm)