Sepax Proteomix POR50-dT20 Affinity Resin is a monodispersed PS/DVB base bead that has a profuse porous structure with large through pores, good physical and chemical stability and good pressure resistance. Oligo dT20-mer is conjugated to the bead surface through a proprietary method. The resulting affinity resin is specially designed and highly optimized for the isolation of messenger RNA (mRNA).
Most mRNA molecules contain a tail of poly(adenylic acid) (polyA tail), up to 250 bases in length. The Proteomix POR dT20 Affinity Resin surface allows capture of mRNA through base pairing between oligo dT20-mer and the mRNA polyA tail using a simple and convenient chromatography procedure.
Provides efficient capture and release under standard mRNA purification conditions, simplifies subsequent purification steps, and maximizes overall production efficiency
Large pore sizes which enables high loading capacity for large mRNAs, easier elution, and high recovery rate.
Decreases process development time and enhances productivity.
Allows reduction in plasmid DNA and other transcription mix components.
Stable at elevated temperatures for the breakdown of undesired higher order structures, if needed.
Excellent scalability. Available as prepacked columns, semi-prep columns, prep columns, and bulk resin.
Particle Size D50
Average Pore Size
dT20 ligand density
≥2.0 mg/mL resin
Dynamic Binding Capacity Based on Oligo A40
≥0.75 mg/mL resin
Max Linear Flow Rate
Operating Pressure (Process FPLC Condition)
≤10 bar (1 MPa)
Operating Pressure (Analytical HPLC Condition)
≤100 bar (10 MPa)
Mobile Phase Compatibility
Compatible with common salt buffers, organic/aqueous solutions (acetonitrile, ethanol, etc.), and common additives for mRNA purification
Store in 20% ethanol aqueous solution, 2-8℃. Do not freeze resin or column.
0.1-0.5M NaOH. Recommend starting with 0.1M NaOH to prolong resin life
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Proteomix POR50-dT20, Pre-Packed Column (7.5 x 100 mm, 4.2mL)