Sepax Monomix dT20 Affinity Resin is a narrowly dispersed polymethacrylate rigid base bead, functionalized with a polyhydroxylated surface coating layer that provides a bio-inert surface, which shows low non-specific binding. Oligo dT20-mer is then conjugated to the bead surface through a proprietary method.
The resulting affinity resin is specially designed and highly optimized for the isolation of messenger RNA (mRNA). Most mRNA molecules contain a tail of poly(adenylic acid) (polyA tail), up to 250 bases in length. The Monomix dT20 Affinity Resin surface allows capture of mRNA though base pairing between oligo dT20-mer and the mRNA polyA tail using a simple and convenient chromatography procedure.
Provides efficient capture and release under standard mRNA purification conditions, simplify subsequent purification steps and maximize overall production efficiency.
Decreases process development time and enhances productivity.
Allows reduction in plasmid DNA and other transcription mix components.
Stable at elevated temperatures for the breakdown of undesired higher order structures, if needed.
Excellent scalability. Provide prepacked columns, semi-prep column, prep column and bulk resin.
Non-animal derived
Resin customization is available. Polymethacrylate bead size is available at 10, 15, 30 and 60µm
Technical Specifications
Resin Type
Monomix dT(20)
Base Matrix
Hydrophilic polymethacrylate
Particle Size D50 (µm)
30
Average Pore Size (Å)
1000
Functional Group
Oligo dT20-mer
Ligand Density
≥2.1 mg dT20 / mL of resin
mRNA Binding Capacity (mg/ml resin)
≥2.0 mg
Max Linear Flow Rate (cm/hr)
1000
Operating Temperature (℃)
4-65
pH Stability
2-12
Operating Pressure
≤100 bar (10 MPa)
Mobile Phase Compatibility
Compatible with common salt buffers, organic/aqueous solutions (acetonitrile, ethanol, etc.), and common additives for mRNA purification
Long-term Storage
Store in 20% ethanol aqueous solution, 2-25℃. Do not freeze resin or column.
CIP
0.1-0.5M NaOH. Recommend to start with 0.1M NaOH to prolong resin life
Support Matrix: Monomix 1000 Å
Average Particle Size: 30 µm
Surface Functionality/Ligand: Oligo dT20
Binding Capacity: >2.0 mg mRNA per mL of resin
pH Range: 2-12
Operating Backpressure/Mechanical Stability: 100 Bar (10 MPa)
Sepax mRNA 2022